An initial denaturation step for 3 min at 95 °C was followed by 35 cycles of denaturation for 30 s at 95 °C, annealing for 30 s at 50 °C, and extension for 1 min at 72 °C, with a final extension step of 10 min at 72 °C. Verticillium lecanii. The causal organism is Cephaleuros virescens, a green parasitic alga whose usual hosts are plants with leathery leaves such as cotoneasters, magnolias, hollies, rhododendrons and viburnums. © 2021 Springer Nature Switzerland AG. Copper Oxychloride is for the control of fungal and bacterial diseases in fruit and vegetable crops, citrus, stone fruit, pome fruit… Read More. This confirms C. virescens as a causal organism of algal leaf spot disease on rambutan in southern Thailand. Leaf tissue may die beneath the spots and the leaves may yellow and drop prematurely. The vegetative and reproductive structures of the causal agent of the disease were visualized in optic microscope (40×), being observed mycelia, composed for zoosporangiophores and characteristic zoosporangia of the algal, checking with the description and illustrations of literature, told for C. virescens Kunze. The PCR performed in 50 μl reaction volume containing 10 pmol of each primer, 2x DreamTaq Green PCR Master Mix (Thermo Scientific), and 50 ng of template DNA. On leaves of camellia and southern magnolia, the lichen Strigula appears as numerous small gray-white crusty spots (figure 4), which later turn yellow and are shed. Macrophoma rot Macrophoma mangiferae syringae; P. cichorii. The disease does not cause severe economic loss. is a large- sized, evergreen tree belonging to … Bryopsis plumose represents the lack of similarity outside this group. Algal leaf spot is sometimes called green scurf because the spots may have a crusty, fuzzy or flaky appearance. Site Feedback @2020 Plant Pathology Department University of Florida Institute of Food and Agricultural Sciences. This confirms C. virescens as a causal organism of algal leaf spot disease on rambutan in southern Thailand. ... Related: Algal Leaf Spot Of Guava: Symptoms And Management. Black spot is the most serious disease on roses in Florida. Hendersonia rot Hendersonia creberrima. They vary in color from a crusty gray-green to greenish brown. Like other plants, Para rubber is attacked by several pathogens and one of the most frequently found is a leaf spot caused by a phytoparasitic algae. Phaeosphaerella mangiferae Phoma sorghina Pseudocercospora mali Pseudocercospora subsessilis Septoria sp. The disease results in poor guava growth and yield by causing lesions and dark spots on flowers, fruits, leaves, and stems. Symptoms of the disease occurred on the leaves and fruit. Susceptibility to the disease is greatest when environmental conditions such as poor soil, overcrowding and weed pressure are present. In this study Cephaleuros virescens was found to be the causal organism of a leaf spot disease on Para rubber (Hevea brasiliensis). It is commonly cultivated in tropical and sub-tropical areas and is an economically important source of natural latex rubber. Specimens of algae have also been deposited at the Culture Collection of the Pest Management Department, Faculty of Natural Resources, Prince of Songkla University, Thailand, for further studies. This field is for validation purposes and should be left unchanged. In this study Cephaleuros virescens was found to be the causal organism of a leaf spot disease on Para rubber (Hevea brasiliensis).The algae caused circular orange to dark brown lesions on both leaf surfaces. Plant Dis- PD-43, Pitaloka MK, Petcharat V, Sunpapao A (2014) Cephaleuros solutus Karsten, as a causal agent of durian (Durio zibethinus Murray) algal leaf spot disease in Thailand. The morphology of the algae (thallus, filamentous cells, sporangiophore, sporangia, gametangia and zoospore) were examined and found to consistently match the species Cephaleuros virescens. The disease is called algal leaf spot, algal fruit spot, and green scurf; Cephaleuros infections on tea and coffee plants have been called “red rust.” These are aerophilic, filamentous green algae. Academic, San Diego, pp 315–322. The symptoms on leaf tissues were then photographed. Various genotypes of M. rosae, i.e isolates that infect a specific cultivar or group of cultivars, are currently present. Algal thalii were selected from fresh leaves and initially observed under a stereomicroscope. Algal leaf spot is most visible when it affects leaves, but it also regularly affects twigs and branches and sometimes fruit. Algal leaf spot is a foliar disease most commonly seen in warm, humid climates or in greenhouses. This nucleotide sequence was deposited in GenBank with accession number (AB984776) and compared to known Cephaleuros and other algal genus on the NCBI (the National Center for Biotechnology Information) databases. Groups of Plant Pathogens – Algae (contd…) https://doi.org/10.1007/s13314-015-0158-1, DOI: https://doi.org/10.1007/s13314-015-0158-1, Over 10 million scientific documents at your fingertips, Not logged in Based on the symptoms observed and microscopic morphological studies, the recovered pathogen was identified as Cephaleuros virescens . It has been previously reported that algal leaf spot disease on rubber plants in Sri Lanka is caused by Cephaleuros parasiticus (Karst) (Munasinghe 1961; Jayasinghe 2001 ). A 94 % sequence identity confirmed it to be a member of the Cephaleuros group. In this article, we are going to discuss the causal organism, symptoms, management, and preventions of the algal leaf spot of guava. Bootstrap values are shown on the branches and the GenBank accession number are shown in parentheses. Algal leaf spot is a foliar disease most commonly seen in warm humid climates or in greenhouses. A PCR product band about 700 bases was observed from Cephaleuros sample. Google Scholar, Hibbet DS (1996) Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi. Neighbor joining tree showing the genetic relationships of Thai Cephaleuros (PSU-PR01) was in the same clade with Cephaleuros genus, while well separated from the other genus (Fig. Although aerophilic and ter-restrial, they require a film of water to complete their life cycles. The purpose of our study was to identify and characterize Cephaleuros species present on Para rubber in Thailand. This is also a fungal disease that affects the fruit and growth … The PCR products were visualized by agarose gel electrophoresis. Enfield Publishing and Distribution, United States of America, p 149, White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Leaf blight Bipolaris hawaiiensis. To confirm the result from morphological characteristics, thallus of Cephaleuros was cultured on Bold’s basal medium (Bischoff and Bold 1963; Andersen 2005) and subjected to DNA extraction and PCR amplification. During September 2015, we observed orange to dark-brown algal leaf spots on leaves of Manilkara zapota (L.) P. Royen (sapodilla, lamoot) in Chiang Mai Province, Thailand. During 2014–2015, algal spot disease was noticed in the guava orchards of northern and southern Thailand. Abstract Guava (Psidium guajava Linn.) Leaf spot Curvularia lunata Leptosphaeria sp. 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